Although the available data on soil NO emission are increasing, only few measurements have been made in temperate forests. Emissions from these forests are generally low, but recent studies have shown that NO emission from N-affected forests can be much higher. In this study the emission of NO from the floor of a moderately N-affected Norway spruce forest in Denmark was measured using dynamic chambers during two seasons (April 1995 and September 1995). At the same time, fluxes of CO2 and NO2, soil water content inside and outside the chambers, soil temperature at 10 cm depth, soil surface temperature and air temperature were recorded. The emission of NO in April was low (<0.3 ng N m-2 s-1), whereas it was significantly higher in September (21 ng N m-2 s-1 with a range 0--82 ng N m-2 s-1). In April the soil surface temperature was low (<6 ¿C) and the soil moisture was high (26% vol/vol). In September the soil surface temperature was high (16 ¿C) and soil moisture was significantly lower (14% vol/vol) than in April. There was a high correlation between CO2 emission and NO emission. The fluxes of NO2 were very small and not related to NO emission. Generally, the variation within a single plot was small, whereas the variation between plots was large. About half of the total variation of NO emission in September could be explained by a combination of soil moisture, distance to tree trunks, and moss cover. No significant relationship could be found between NO emission and soil surface temperature or soil temperature. Other parameters such as the C/N dynamics in the soil must be taken into account to provide a better mechanistic understanding of the NO emission. An annual emission of 3.15 kg NO-N ha-1 yr-1 was estimated by the amount of NH4+ in throughfall. Compared to most of the previous published measurements of NO emission from temperate forest soils, the values presented here are high and of the same magnitude as found in N-affected forests in Central Europe. ¿ 1999 American Geophysical Union