Daytime and nighttime vertical profiles of the tropospheric trace gas N2O were determined from grab sample collections off the Atlantic and Gulf coasts of Florida. The grab samples were collected during the the week of October 7--13, 1984, from a Lear Jet during descent spirals over an altitude range of 12.5--0.3 km in approximately 1.2-km intervals. During this period there were two distinct airflow regimes sampled: the surface boundary layer (<2 km), in which the wind direction was typically easterly; and the regime above the boundary layer, which was predominantly characterized by westerly flow. N2O mixing ratios, normalized to dry air, were determined from 148 daytime and nighttime samplings. N2O was found to be uniformly mixed at all altitudes at 301.9¿2.4 parts per billion by volume.