Ground-based microwave spectroscopy measurements of mesospheric CO and H2 O vertical mixing ratio profiles are used to infer vertical mixing rates in the upper mesosphere. The CO and H2 O data consistently imply vertical eddy diffusion coefficients in the 70- to 85-km region of ≲(1--2)¿105 cm2 s-1 during spring through summer at mid-latitudes. Although chemical acceleration of vertical transport is substantial for O and O3 below the mesopause, the divergences of their associated fluxes are modest, with at most a factor of 2 effect on the concentrations of O and O3 for measured variability in gravity wave activity. Comparison of Solar Mesosphere Explorer (SME) O3 data with model results reinforces the conclusions of slow vertical mixing in the upper mesosphere as a consequence of the reduced HOx catalytic loss of odd oxygen. The changes in chemical rate constants recommended by Rusch and Eckman (1985), in conjunction with slow vertical mixing, yield good agreement with SME O3 data. The slow vertical mixing deduced in this study is consistent with upper limits obtained from studies of the mesospheric heat budget and could be construed as evidence for an advectively controlled mesosphere. A comparison of the vertical eddy diffusion coefficients for momentum stresses, constituent transport, and heat transport suggests that the eddy Prandtl number must be of order 10. ¿American Geophysical Union 1987