Tropical deep convection and its dynamical effect on the tropopause and stratosphere are investigated using a suite of data from the Upper Atmospheric Research Satellite (UARS) Microwave Limb Sounder (MLS), including upper tropospheric humidity, cloud radiance, and gravity wave measurements. For this purpose, geographical distributions of temperature, water vapor, and cloudiness in the tropical tropopause layer (TTL) are compared with corresponding maps of gravity wave variance in the stratosphere. In addition, ECMWF global wind divergent and velocity potential fields as well as NOAA outgoing longwave radiation and CMAP rainfall data are analyzed to help pinpoint the locations of deep convection. We found that high-altitude clouds near the bottom of TTL (~147 hPa) are usually surrounded by high-humidity air, and their spatial pattern and seasonal variability are closely associated with regions of vigorous summertime deep convection. Upward propagating gravity waves generated from these convection regions are shifted poleward by prevailing stratospheric winds. We estimate that tropical deep convection lifts ~5% of the cloud tops to altitudes above 100 hPa and that most of the extreme deep convection events occur in the Western Pacific and Indian monsoon regions. Low-temperature regions in the TTL are associated with, but often drift away from, the center of deep convection. Regions of water vapor maxima near the bottom of TTL are located directly above the deep convection centers, but this moisture behavior is somewhat reversed at the top of the TTL. The integrated picture derived from this study implies that convective scale motions could be important in affecting short-term dehydration processes in the TTL. Our results also suggest that the spatial organization and temporal development of tropical convective systems will be better monitored with the follow-on Earth Observing System (EOS) Aura satellite instruments and lead to improved understanding of the complex interaction of tropical convection with large-scale dynamic and thermodynamic conditions.