Vibrational excitation of ground-state NO through collisions with oxygen atoms produces NO(v = 1) in the lower thermosphere, representing a significant source of atmospheric cooling through the subsequent 5.3-¿m radiative emission. A laser pump-probe experiment has been used to measure the temperature dependence of the NO(v = 1)-O vibrational relaxation rate coefficient kO(v = 1) in the 295--825 K range, along with updated measurements of kO(v = 1,2) at room temperature. The experiment employed a continuous wave microwave source to form O atoms, combined with photolysis of a trace amount of added NO2 to produce vibrationally excited NO. Oxygen atoms were detected through two-photon laser-induced fluorescence, cross-calibrated against a normalized O atom signal resulting from photolysis of a known concentration of NO2. No temperature dependence was observed for kO(v = 1) to within the uncertainty in the measurements. The measured room temperature value of kO(v = 1) = (4.2 ¿ 0.7) ¿ 10-11 cm2 s-1 is 75% larger than the value obtained previously in this laboratory, a significant difference at the 1σ level. The present value is preferred owing to an improved experimental technique. The atmospherically relevant NO(v = 0)-O vibrational excitation rate coefficient can be derived from measured values of kO(v = 1) through detailed balance. The variable temperature measurements provide key information for aeronomic models of the lower thermospheric energy budget, infrared emission intensities, and neutral constituent densities.