This study examined daily streamflow response over up to four decades in northwest conifer forest and eastern deciduous forest sites in the United States. We used novel methods to analyze daily observations of climate and streamflow spanning more than 900 basin years of record at 14 treated/control basin pairs where forest removal and regrowth experiments were underway in the period 1930--2002. In the 1 to 5-year period after forest removal, maximum daily increases ranged from 2 to 3 mm at deciduous forest sites, to 6 to 8 mm at conifer forest sites. Significant spring surpluses persisted for up to 35 years in conifer forest basins, but winter and spring streamflow deficits appeared after 10 to 15 years of forest regrowth in eastern deciduous forest basins. In all 5-yr posttreatment periods, absolute changes in daily streamflow were significantly more likely during moist, warm seasons, or during snowmelt seasons, but relative changes were more likely during warm seasons irrespective of moisture status. Both relative and absolute streamflow changes in the 1 to 5 and 15 to 25-year periods after forest removal were significantly positively related to the age of the forest at the time it was cut. Eastern deciduous forests had been disturbed by logging or hurricane 12 to 56 years prior to forest removal, while Pacific Northwest conifer forests had been not experienced logging or wildfire for 90 to 450 years. Paired basin experiments provide a continuous, and continuously changing, record of vegetation structure, composition, and climate, and their effects on streamflow.