This paper incorporates the latest improvements in intersatellite calibration, along with a new statistical technique, to determine the diurnal and seasonal cycles and climatic trends of 1978--2004 tropospheric temperature using Microwave Sounding Unit measurements. We also compare the latitudinal distribution of temperature trends from the surface and troposphere with each other and with model simulations for the past 26 years. The observations at the surface and in the troposphere are consistent with climate model simulations. At middle and high latitudes in the Northern Hemisphere, the zonally averaged temperature at the surface increased faster than in the troposphere while at low latitudes of both hemispheres the temperature increased more slowly at the surface than in the troposphere. The resulting global averaged tropospheric trend is +0.20 K/10 yr, with a standard error of 0.05 K/10 yr, which compares very well with the trend obtained from surface reports.