The ability to accurately validate high--spectral resolution infrared radiance measurements from space using comparisons with a high-altitude aircraft spectrometer has been successfully demonstrated. The demonstration is based on a 21 November 2002 underflight of the AIRS on the NASA Aqua spacecraft by the Scanning-HIS on the NASA ER-2 high-altitude aircraft. A comparison technique which accounts for the different viewing geometries and spectral characteristics of the two sensors is introduced, and accurate comparisons are made for AIRS channels throughout the infrared spectrum. Resulting brightness temperature differences are found to be 0.2 K or less for most channels. Both the AIRS and the Scanning-HIS calibrations are expected to be very accurate (formal 3-sigma estimates are better than 1 K absolute brightness temperature for a wide range of scene temperatures), because high spectral resolution offers inherent advantages for absolute calibration and because they make use of high-emissivity cavity blackbodies as onboard radiometric references. AIRS also has the added advantage of a cold space view, and the Scanning-HIS calibration has recently benefited from the availability of a zenith view from high-altitude flights. Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations, including those from the NASA EOS spacecraft (Terra, Aqua and Aura) and the new complement of NPOESS operational instruments. The validation role for accurately calibrated aircraft spectrometers also includes application to broadband instruments and linking the calibrations of similar instruments on different spacecraft. It is expected that aircraft flights of the Scanning-HIS and its close cousin the NPOESS Airborne Sounder Test Bed (NAST) will be used to check the long-term stability of AIRS and the NPOESS operational follow-on sounder, the Cross-track Infrared Sounder (CrIS), over the life of the missions.