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Detailed Reference Information |
Bernhard, J.M., Ostermann, D.R., Williams, D.S. and Blanks, J.K. (2006). Comparison of two methods to identify live benthic foraminifera: A test between Rose Bengal and CellTracker Green with implications for stable isotope paleoreconstructions. Paleoceanography 21: doi: 10.1029/2006PA001290. issn: 0883-8305. |
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The conventional method to distinguish live from dead benthic foraminifers uses Rose Bengal, a stain that reacts with both live and dead cytoplasm. CellTracker Green CMFDA is a fluorogenic probe causing live cells to fluoresce after proper incubation. To determine the more accurate viability method, we conducted a direct comparison of Rose Bengal staining with CellTracker Green labeling. Eight multicore tops were analyzed from Florida Margin (SE United States; 248--751 m water depths), near Great Bahama Bank (259--766 m), and off the Carolinas (SE United States; 220 and 920 m). On average, less than half the Rose Bengal--stained foraminifera were actually living when collected. Thus, while Rose Bengal can significantly overestimate abundance, combined analyses of CellTracker Green and Rose Bengal can provide insights on population dynamics and effects of episodic events. Initial stable isotope analyses indicate that the CellTracker Green method does not significantly affect these important paleoceanographic proxies. |
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Abstract |
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Keywords
Paleoceanography, Micropaleontology (0459, 3030), Paleoceanography, Instruments and techniques, Biogeosciences, Biosignatures and proxies |
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Publisher
American Geophysical Union 2000 Florida Avenue N.W. Washington, D.C. 20009-1277 USA 1-202-462-6900 1-202-328-0566 service@agu.org |
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