We screened 51 species of trees and vines for isoprene emission by using a tower crane to gain access to the top of the canopy in a semideciduous forest in the Republic of Panama. Of the species screened, 15 emitted isoprene at rates greater than 0.8 nmolm-2s-1. We measured the influence of light and temperature on emissions. The species-dependent emission rates at 303 K and 1000 &mgr;mol m-2s-1 of incident photosynthetically active radiation ranged from 9 to 43 nmolm-2s-1 with coefficients of variation of about 20%. Isoprene emission showed a hyperbolic response to light intensity and an exponential response to temperature. We modified an existing algorithm developed for temperate plants to fit the temperature response of these tropical species. We suggest a new algorithm to fit the light response of isoprene emission. The new and modified algorithms are compared to the algorithms developed for temperate plants that are used in global models of isoprene emission. Both sets of algorithms also are compared to additional validation data collected in Panama and to published data on isoprene emission from a tropical dry forest in Puerto Rico. Our comparisons suggest that algorithms developed for temperate plants can significantly underestimate isoprene emissions from tropical forests at high-light and high-temperature levels. ¿ 1999 American Geophysical Union