Measurements of H2O in the tropopause region have been obtained by production of a new data set from the Microwave Limb Sounder (MLS) on the Upper Atmosphere Research Satellite (UARS). A modified version of the retrieval scheme used to produce upper tropospheric humidity (UTH) from the MLS 203 GHz radiometer was applied to the MLS 183 GHz radiometer measurements to produce useful H2O data at 147, 121, 100, 83, 68, and 56 hPa. These new data, for the first 18 months of the UARS mission when the MLS 183 GHz radiometer was operational, fill an important gap around 100 hPa where previous MLS H2O data were generally not useful. Characteristics of the new data set are discussed and compared with National Oceanic and Atmospheric Administration (NOAA), Climate Monitoring and Diagnostics Laboratory (CMDL) frost-point hygrometer, and UARS Halogen Occultation Experiment (HALOE) measurements.